PlastiPure is able to evaluate and remediate a wide variety of items which may leach chemicals with estrogenic activity (EA), including most consumer products, packaging, cosmetics, plastics and other materials, additives, colorants, chemicals, and more.
PlastiPure can use its molecular models and large EA database to predict whether a sample is likely to have EA. While this initial review is not definitive, it often provides basic information to assist in determining next steps in evaluating and remediating products.
Sample Stressing and Extraction
PlastiPure uses a variety of stress protocols and food simulants to prepare samples for testing. Chemicals extracted under these conditions represent some of the potentially hazardous compounds that might be released during lifetime use of the product. These stresses include moist heat for dishwashing, UV for sunlight, microwaving, boiling, unstressed, and other conditions. Generally, mixtures of water and ethanol in different concentrations are used to extract for a variety a potential foods, drinks, or cosmetics.
Testing for Estrogenic Activity
After stressing and extraction, PlastiPure works closely with its sister company, CertiChem, to utilize CertiChem’s highly sensitive robotized cell-based assays for EA detection. CertiChem tests the samples prepared by PlastiPure and measures EA relative to a negative control (e.g., water or ethanol) and a known estrogen positive control (e.g., 17 beta estradiol). Generally, a sample is deemed positive, if it the EA levels are significantly higher than the negative control at a given relative concentration. Test results are validated by a CertiChem confirmation assay.
Support and Validation for Robotized EA Testing
CertiChem has developed its automated bioassays over 14 years, with funding and collaboration from multiple U.S. federal agencies including the National Institute of Environmental Health Sciences (NIEHS), the National Science Foundation (NSF), the National Toxicology Program (NTP), and Interagency Coordinating Committee on the Validation of Alternative Methods / NTP Interagency Center for the Evaluation of Alternative Toxicological Methods (ICCVAM/NICEATM).
CertiChem’s testing methods and effectiveness have been published in multiple peer-reviewed papers and have resulted in millions of dollars in grant and government funding for ongoing development. CertiChem has robotized two primary cell-based assays based on the BG1-Luc (validated by ICCVAM and OECD) and the MCF-7 (undergoing ICCVAM validation) cell lines.
Testing can generally be performed within 3-6 weeks from receipt of the sample. The actual time it takes is dependent on stressing and extracting protocols (3 days – 3 weeks), cell line used (3 days – 2 weeks), and our current testing queue.